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dc.contributor.authorArnoldussen, Yke Jildouw
dc.contributor.authorErvik, Torunn Kringlen
dc.contributor.authorSamulin-Erdem, Johanna Maria
dc.contributor.authorKero, Ida
dc.contributor.authorEriksen, Mina Baarnes
dc.contributor.authorSkaug, Vidar
dc.contributor.authorZienolddiny, Shanbeh
dc.date.accessioned2019-12-05T09:09:28Z
dc.date.available2019-12-05T09:09:28Z
dc.date.created2019-02-15T13:01:51Z
dc.date.issued2019
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/11250/2631875
dc.description.abstractTremendous efforts are applied in the ferroalloy industry to control and reduce exposure to dust generated during the production process, as inhalable Mn-containing particulate matter has been linked to neurodegenerative diseases. This study aimed to investigate the toxicity and biological effects of dust particles from laboratory-scale processes where molten silicomanganese (SiMn) was exposed to air, using a human astrocytoma cell line, 1321N1, as model system. Characterization of the dust indicated presence of both nano-sized and larger particles averaging between 100 and 300 nm. The dust consisted mainly of Si, Mn and O. Investigation of cellular mechanisms showed a dose- and time-dependent effect on cell viability, with only minor changes in the expression of proteins involved in apoptosis. Moreover, gene expression of the neurotoxic biomarker amyloid precursor protein (APP) increased, whereas APP protein expression decreased. Finally, induction of gap junctional intercellular communication (GJIC) increased with higher doses and correlated with the other endpoints. Thus, the effects of SiMn dust on 1321N1 cells are highly dependent on the dose of exposure and involves changes in APP, apoptosis-related proteins and intercellular communication.
dc.language.isoeng
dc.titleMechanisms of Toxicity of Industrially Relevant Silicomanganese Dust on Human 1321N1 Astrocytoma Cells: An In Vitro Study
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.volume20
dc.source.journalInternational Journal of Molecular Sciences
dc.source.issue3
dc.identifier.doi10.3390/ijms20030740
dc.identifier.cristin1677676
cristin.unitcode7476,0,0,0
cristin.unitnameStatens arbeidsmiljøinstitutt
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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