dc.contributor.author | Camassa, Laura Maria Azzurra | |
dc.contributor.author | Ervik, Torunn Kringlen | |
dc.contributor.author | Zegeye, Fikirte Debebe | |
dc.contributor.author | Mdala, Ibrahimu | |
dc.contributor.author | Valen, Håkon | |
dc.contributor.author | Ansteinsson, Vibeke Elise | |
dc.contributor.author | Zienolddiny, Shanbeh | |
dc.date.accessioned | 2024-08-19T07:10:07Z | |
dc.date.available | 2024-08-19T07:10:07Z | |
dc.date.created | 2021-04-28T10:44:35Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Dental Materials. 2021, 37 (7), 1121-1133. | |
dc.identifier.issn | 0109-5641 | |
dc.identifier.uri | https://hdl.handle.net/11250/3146868 | |
dc.description.abstract | Objective The project aims to evaluate whether inhalation of particles released upon grinding of dental composites may pose a health hazard to dentists. The main objective of the study was to characterize the dust from polymer-based dental composites ground with different grain sized burs and investigate particle uptake and the potential cytotoxic effects in human bronchial cells. Methods Polymerized blocks of two dental composites, Filtek™ Z250 and Filtek™ Z500 from 3M™ ESPE, were ground with super coarse (black) and fine (red) burs inside a glass chamber. Ultrafine airborne dust concentration and particle size distribution was measured real-time during grinding with a scanning mobility particle sizer (SMPS). Filter-collected airborne particles were characterized with dynamic light scattering (DLS) and scanning electron microscopy (SEM). Human bronchial epithelial cells (HBEC-3KT) were exposed to the dusts in dose-effect experiments. Toxicity was measured with lactate dehydrogenase (LDH) assay and cell counting kit-8 (CCK8). Cellular uptake was observed with transmission electron microscopy (TEM). Results Airborne ultrafine particles showed that most particles were in the size range 15−35 nm (SMPS). SEM analysis proved that more than 80% of the particles have a minimum Feret diameter less than 1 μm. In solution (DLS), the particles have larger diameters and tend to agglomerate. Cell toxicity (LDH, CCK8) is shown after 48 h and 72 h exposure times and at the highest doses. TEM showed presence of the particles within the cell cytoplasm. | |
dc.description.abstract | Characterization and toxicity evaluation of air-borne particles released by grinding from twodental resin composites in vitro | |
dc.language.iso | eng | |
dc.title | Characterization and toxicity evaluation of air-borne particles released by grinding from twodental resin composites in vitro | |
dc.title.alternative | Characterization and toxicity evaluation of air-borne particles released by grinding from twodental resin composites in vitro | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 1121-1133 | |
dc.source.volume | 37 | |
dc.source.journal | Dental Materials | |
dc.source.issue | 7 | |
dc.identifier.doi | 10.1016/j.dental.2021.03.011 | |
dc.identifier.cristin | 1906881 | |
dc.relation.project | Norges forskningsråd: 288768 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |